PriCells: Recombinant Human Granulocyte-Macrophage Colony Stimulating   
                Factor (GM-CSF)

Granulocyte-macrophage colony-stimulating factor, often abbreviated to GM-CSF, is a protein secreted by macrophages, T cells, mast cells, endothelial cells, and fibroblasts. GM-CSF contains N-linked and possibly O-linked oligosaccharides.

GM-CSF functions
GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. GM-CSF is known as molgramostim or, when the protein is expressed in yeast cells, sargramostim (Leukine). GM-CSF is used as a medication to stimulate the production of white blood cells following chemotherapy. GM-CSF has also recently been evaluated in clinical trials for its potential as a vaccine adjuvant in HIV-infected patients. The preliminary results have been promising but GM-CSF is not presently FDA-approved for this purpose.

Theoretical Sequence:
APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE

Purify: 95%, as determined by SDS-PAGE analysis

Species: Human

Product Size: 1 μg , 10 μg . 100 μg 

Purify Grade: Carrier-Free

Protein Family: Growth Factors

Endotoxin Level: <1EU/ml

Molecular Weight: GM-CSF migrates as a broad band between 15 and 30 kDa on SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with unmodified GM-CSF that has a predicted molecular mass of 14.5 kDa. Purified GM-CSF consists of 5-50% carbohydrate by weight.

Production: rhGM-CSF is produced in E. coli and purified via sequential chromatography. Filtered prior to lyophilization through a 0.22 micron sterile filter.

Biological Activity: ED50 range = 0.02 to 0.1 ng/mL, determined by the dose dependent proliferation of human TF-1 cells. Optimal concentration for individual application should be determined by a dose response assay.

Negative test: HIV-1, HBV, HCV, Mycoplasmas, Bacteria, yeast, and fungus

Protein Form: recombinant human

Expression and Purification: rhGM-CSF is expressed in E. coli and purified via sequential chromatography.

Contents and Storage: Store this lyophilized preparation at -20°C, preferably desiccated. Upon reconstitution, apportioninto working aliquots and store at -20°C. Avoid repeated freeze/thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

These products are for research use only and are not intended for human or animal diagnostic, or therapeutic.

Shipping: Products may be shipped on ice pack or dry ice.