PriCells: Recombinant Human Macrophage Colony Stimulating Factor (M-CSF)

Macrophage-colony stimulating factor (M-CSF) is produced by fibroblasts, endothelial cells, stromal cells, macrophages, osteoblasts and other cell types.

M-CSF is required for growth and differentiation of monocytes and macrophages. M-CSF polarizes macrophages into the M2 phenotype where anti-inflammatory IL-10 is produced, rather than the M1 phenotype where inflammatory cytokines are produced. M-CSF also recruits monocytes and enhances angiogenesis by inducing VEGF production.

M-CSF binds to its receptor (CSF1R); downstream signaling involves PI3K/Akt, ERK and STATs 1, 3, and 5. An increase in M-CSF expression may contribute to cancer progression and a higher plasma M-CSF level is associated with rheumatoid arthritis.

Purify: 95%, as determined by SDS-PAGE analysis

Species: Human

Product Size: 1μg , 10μg , 100μg 

Purify Grade: Carrier-Free

Protein Family: Growth Factors

Endotoxin Level: <1EU/ml

Molecular Weight: 36.8 kDa

Protein Length or Portion (a.a.): 159 aa (homodimer)

Production: rhM-CSF is produced in E. coli and purified via sequential chromatography. Filtered prior to lyophilization through a 0.22 micron sterile filter.

Biological Activity: ED50 < 1.0 ng/mL (specific activity > 1 x 10⁶ units/mg). The biological activity was determined by measuring the dose dependent stimulation of proliferation of mouse M- NFS-60 cells. The optimal concentration should be determined for each specific application.

Negative test: HIV-1, HBV, HCV, Mycoplasmas, Bacteria, yeast, and fungus

Protein Form: recombinant human

Expression and Purification: rhM-CSF is expressed in E. coli and purified via sequential chromatography.

Contents and Storage: Store this lyophilized preparation at -20°C, preferably desiccated. Upon reconstitution, apportioninto working aliquots and store at -20°C. Avoid repeated freeze/thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

These products are for research use only and are not intended for human or animal diagnostic, or therapeutic.

Shipping: Products may be shipped on ice pack or dry ice.